孟清
发布部门:化学化工与生物工程学院       发布时间:2020-07-13



 

孟清,理学博士(毕业于中山大学生物化学与分子生物学专业),加拿大Dalhousie大学医学院博士后,美国康内尔大学医学院高级研究学者,瑞典卡罗林斯卡医学院、瑞典农业大学客座教授,新加坡国立大学客座教授,中国生物化学与分子生物技术专业委员会理事,上海市学位委员会学科评议组成员,上海市生物化学与分子生物学会理事,上海市细胞学会理事,东华大学生物化学与分子生物学、基因工程仿生生物材料学教授,博士生导师。


研究方向

1.传染性病原菌和传染性病毒检测技术和疫苗的研发

2.编码蜘蛛丝蛋白质基因的克隆、表达、结构与功能、成丝机理及仿生研究

3.功能基因中蛋白质内含子的剪接机理与应用研究

4.原核生物基因内含子的结构与功能、剪接与转移机理的研究


主要成就

1.主持完成国际合作、国家自然科学基金(项目编号:39460047)等28项科研项目。

2.发表研究论文100多篇

3.1986年获一项中国科学院科技进步一等奖,1996年获一项中国农业部科技进步三等奖

4.出版专著一部


近年来主持的主要科研项目

1.上海市浦江人才计划研究项目(项目编号:05PJ14015),2005-2007

2.国家高技术研究发展计划(863计划)项目(项目编号:2006AA03Z451),2006-2009

3.国家自然科学基金面上项目(项目编号:30770463),2008-2010

4.国家自然科学基金面上项目(项目编号:31070698),2011-2013

5.上海市基础研究重点项目(项目编号:10JC1400300),2010-2012

6.教育部直属高校聘请外籍教师重点项目,2007-2008

7.教育部直属高校聘请外籍教师重点项目,2009-2010

8.上海市白玉兰人才基金项目(项目编号:2007B004),2007-2008

9.上海市白玉兰人才基金项目(项目编号:2009B009),2009-2010

10.教育部博士点基金项目(课题编号:20120075110007),2013-2015

11.教育部直属高校聘专项目(1), 2013-2014

12.教育部直属高校聘专项目(2), 2013-2014

13.教育部直属高校学校特色项目(课题编号:TS2011DHDX025),2013-2015

14.上海市外聘专家项目,2013-2014

15.国家外专局“高端外国专家项目”,(课题编号:GDW20143100069),2014-2016

16.国家外专局“高端外国专家项目”,(课题编号:GDW20143100070),2014-2016

17.国家外专局“高端外国专家项目”,(课题编号:GDW20143100071),2014-2016

18.上海市科委攻关项目,(课题编号:14521100700),2014-2017

19.上海市科委国际合作项目,(课题编号:14520720200),2014-2017

20.国家自然科学基金面上项目(项目编号:31570721),2016-2019

21.军委科技委项目(项目编号:17-163-12-ZT-003-86-01),2017-2018

22.蜘蛛丝项目,2019.01-2020.12 


近年来发表的主要研究论文(*表示通讯作者)

1.Zhu H1, Rising A2, Johansson J3, Zhang X4, Lin Y1, Zhang L1, Yi T1, Mi J1, Meng Q*. Tensile properties of synthetic pyriform spidersilk fibers depend on the number of repetitive units as well as the presence of N- and C-terminal domains. Int J Biol Macromol. 2020 Mar 10. S0141-8130(20)30691-7(SCI,影响因子:4.7)

2.Yizhong Zhou, Qingchun Shen, Ying Lin, Shouying Xu, Qing Meng*。Evaluation of the potential of chimeric spidroins/poly(L-lactic-co-ε-caprolactone) (PLCL) nanofibrous scaffolds for tissue engineering,Materials Science & Engineering C, 2020, 111 110752(SCI,影响因子:4.7)

3.Wang K1, Wen R1, Wang S1, Tian L1, Xiao J1, Meng Q*. The molecular structure of novel pyriform spidroin (PySp2) reveals extremely complex central repetitive region. Int J Biol Macromol. 2020 Feb 15;145:437-444(SCI,影响因子:4.7 )

4.Xu S, Li X, Zhou Y, Meng Q*. Structural characterization and mechanical properties of chimeric masp1/flag minispidroins [J]. Biochimie. 2020,168: 251-258(SCI,影响因子:3.362 )

5.Zhang X1,2, Liu XQ2, Meng Q*. Engineered Ssp DnaX inteins for protein splicing with flanking proline residues,Saudi J Biol Sci. 2019 May; 26(4):854-859(SCI,影响因子:2.002)

6.Wang K1, Wen R2, Jia Q3, Liu X4, Xiao J5, Meng Q*. Analysis of the Full-Length Pyriform Spidroin Gene Sequence. [J]. Genes. 2019,10(6): 425(SCI,影响因子:3.3 )

7.Zhou Y, Rising A, Johansson J, Meng Q*. Production and properties of triple chimeric spidroins. [J]. Biomacromolecules. 2018,19(7): 2825-2833(SCI,影响因子: 5.835 )

8.Xu S, Liu Y, Meng Q* and Baolin Wang*. Rab34 small gtpase is required for hedgehog signaling and an early step of ciliary vesicle formation in mouse [J]. J Cell Sci. 2018,131(21): jcs213710(SCI,影响因子:4.401 )

9.Li X, Zhang XL, Cai YM, Zhang L, Lin Y, Meng Q*. Site specific labeling of two proteins in one system by atypical split inteins.Int J Biol Macromol. 2018 Apr 1;109:921-931(SCI,影响因子:2.502)

10.Rui Wen, Kangkang Wang, Xiangqin Liu, Xue Li, Junpeng Mi, Qing Meng*,  Molecular cloning and analysis of the full-length aciniformspidroin gene from Araneusventricosus,Int J Biol Macromol. 2018 Oct 1;117:1352-1360(SCI,影响因子:2.502)

11.Xu L1, Lefèvre T2, Orrell KE, Meng Q1, Auger M2, Liu XQ, Rainey JK. Structural and Mechanical Roles for the C-Terminal Nonrepetitive Domain Become Apparent in Recombinant SpiderAciniformSilk. Biomacromolecules. 2017 Nov 13;18(11):3678-3686(SCI,影响因子:5.835)

12.Wang C1, Li J1, Meng Q2, Wang 3. Three tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse.Dev Biol. 2017 Oct 1;430(1):156-165(SCI,影响因子:4.904)

13.Li J1, Wang C2, Wu C3, Cao T4, Xu G5, Meng Q6*, Wang*. B7.PKA-mediated Gli2 and Gli3 phosphorylation is inhibited by Hedgehog signaling in cilia and reduced in Talpid3 mutant. Dev Biol.2017 Sep 1;429(1):147-157(SCI,影响因子:4.904)

14.Wen R1, Liu X2, Meng Q*. Characterization of full-length tubuliformspidroin gene from Araneusventricosus. Int J Biol Macromol. 2017 Dec;105(Pt 1):702-710.(SCI,影响因子:2.502)

15.Li, X., C.H. Shi, C.L. Tang, Y.M. Cai, and Q. Meng*, The correlation between the length of repetitive domain and mechanical properties of the recombinant flagelliformspidroin. Biology Open. 2017. 6(3): p. 333-339(SCI,影响因子:2.905)

16.Landreh, M., M. Andersson, E.G. Marklund, Q.P. Jia, Q. Meng, J. Johansson, C.V. Robinson, and A. Rising, Mass spectrometry captures structural intermediates in protein fiber self-assembly. Chemical Communications. 2017. 53(23): p. 3319-3322(SCI,影响因子:5.787)

17.Otikovs M, Andersson M, Jia Q, Nordling K, Meng Q, Andreas LB, Pintacuda G, Johansson J, Rising A, Jaudzems K1. Biomimicry of artificial spidersilk spinning assessed by NMR. Angew Chem Int Ed Engl. 2017 Oct 2;56(41):12571-12575(SCI,影响因子:12.730)

18.Andersson, M., Q.P. Jia, A. Abella, X.Y. Lee, M. Landreh, P. Purhonen, H. Hebert, M. Tenje, C.V. Robinson, Q. Meng, G.R. Plaza, J. Johansson, and A. Rising, Biomimetic spinning of artificial spider silk from a chimeric minispidroin. Nature Chemical Biology. 2017. 13(3): p. 262(SCI,影响因子:15.808)

19.Lin S, Chen G, Liu X, Meng Qing*, Chimeric spider silk proteins mediated by intein result in artificial hybrid silks, Biopolymers. 2016, 105(7):385-92 (SCI,影响因子:2.572)

20.Dai X1, Xun Q, Liu XQ*, Meng Q*. Cysteine-free non-canonical C-intein for versatile protein C-terminal labeling through trans-splicing. Appl Microbiol Biotechnol.2015, 99(19): 8151-61(SCI,影响因子:1.879)

21.Xudong Dai, Xiang-Qin Liu*, Qing Meng*, Segmental expression and C-terminal labeling of protein ERp44 through protein trans-splicing, Protein Expression and Purification. 2015, 112 29–36(SCI,影响因子:1.644)

22.Martins Otikovs, Gefei Chen, Kerstin Nordling, Michael Landreh, Qing Meng, Hans Jörnvall,NinaKronqvist, Anna Rising, Jan Johansson and Kristaps Jaudzems. Diversified structural basis of a conserved molecular mechanism,for pH dependent dimerization in spider silk N-terminal domains,Chembiochem. 2015 Aug 17;16(12):1720-4(SCI,影响因子:5.838)

23.Marie-Laurence Tremblay1*, Lingling Xu2*, Thierry Lefèvre, MuzaddidSarker, Kathleen E. Orrell1, Jérémie Leclerc, Qing Meng, Michel Pézolet, Michèle Auger, Xiang-Qin Liu1 & Jan K. Raine, Spider wrapping silk fibre architecture arising from its modular soluble protein precursor, Scientific Reports. 2015, 26; 5: 11502(SCI,影响因子:4.259)

24.Marlene Andersson1*, Gefei Chen2*, Martins Otikovs3, Michael Landreh4, Kerstin Nordling5, Nina Kronqvist5, Per Westermark6, Hans Jörnvall4, Stefan Knight7, Yvonne Ridderstråle1, Lena Holm1, Kristaps Jaudzems3, Mitchell Chesler8, Qing Meng2*, Jan Johansson1,5,9* and Anna Rising 1,5*,CO2 and H+ drive spider silk formation via opposite effects on the terminal domains,PLoS Biology. 2014, 12(8): e1001921(SCI,影响因子:12.469)

25.Nina Kronqvist, Martins Otikovs, Volodymyr Chmyrov, Gefei Chen, Marlene Andersson, Kerstin Nordling, Michael Landreh, MédouneSarr, Hans Jörnvall, Stefan Wennmalm, Jerker Widengren, Qing Meng, Anna Rising, Daniel Otzen, Stefan D. Knight, Kristaps Jaudzems and Jan Johansson,A charge network enables pH driven sequential dimerization and stabilization of the spider silk N-terminal domain,Nature Communications. 2014, 5:3254,(SCI,影响因子:12.124)

26.Changhua Shi, Qing Meng*, and David W. Wood*. Simple bacterial growth assay for the evalution of human thyroid hormone receptor beta mutants, Journal of Molecular Endocrinology. 2014 52, 55-66(SCI,影响因子:3.628)

27.Shi C, Tarimala A, Meng Q, Wood DW. A general purification platform for toxic proteins based on intein trans-splicing,Appl Microbiol Biotechnol. 2014,98(22): 9425-35,(SCI,影响因子:3.280)

28.Lingling Xu, Marie-Laurence Tremblay, Kathleen E. Orrellb, Jérémie Leclerc, Qing Meng*, Xiang-Qin Liu*, Jan K. Rainey*,Nanoparticle self-assembly by a highly stable recombinant spider wrapping silk protein subunit,FEBS Lett. 2013, 587(19): 3273-80(SCI,影响因子:3.601)

29.Ying Lin, Meng-Meng Li, Hui-Ling Song, Ling-Ling Xu, Xing-Mei Qi, Fang Hu, Qing Meng*, and Xiang-Qin Liu*,Protein trans-splicing of multiple atypical split inteins engineered from natural inteins,PLoS One. 2013, 8(4),e59516(SCI,影响因子:4.411)

30.Gefei Chen, Xiangqin Liu, Yunlong Zhang, Senzhu Lin, Zijiang Yang, Jan Johansson, Anna Rising* and Qing Meng*,Full-length minor ampullatespidroin sequence reveals highly variable organization of spider silk genes,PLoS One. 2012, 7(12),e52293(SCI,影响因子:4.411)

31.Lingling Xu, Qing Meng*, and Xiang-Qin Liu*,Silk fibers self-assembled from repetitive domain of spider wrapping silk AcSp1 protein,PLoS One. 2012, 7(11), e50227(SCI,影响因子:4.411)

32.Huiling Song, Qing Meng*, Xiang-Qin Liu*,Protein Trans-Splicing of an Atypical Split Intein Showing Structural Flexibility and Cross-Reactivity,PLoS One. 2012, 7(9),e45355(SCI,影响因子:4.411)

33.Changhua Shi, Qing Meng*and David W. Wood*,A split inteindual ELP system for recombinant protein purification,Appl Microbiol Biotechnol. 2012, 96, 6, 4601-3(SCI,影响因子:3.280)

34.Lingling Xu, Marie-Laurence Tremblay, Qing Meng, Xiang-Qin Liu, Jan K. Rainey,1H, 13C and 15N NMR assignments of the aciniformspidroin(AcSp1) repetitive domain of Argiope trifasciata wrapping silk. Biomol NMR Assign. 2012, 6,2,147-51(SCI,影响因子:1.980)

35.Xingmei Qi, Qing Meng*, Xiangqi Liu*,Spontaneous C-cleavage of a mini-intein without its conserved N-terminal motif A. FEBS Letters. 2011, 585, 2513-2518(SCI,影响因子:3.601)

36.Qi X, Wang J, Meng Q*,Liu XQ*,Alternative Nucleophilic Residues in Intein Catalysis of Protein Splicing,Protein Pept Lett. 2011, 18, 12, 1226-32(SCI,影响因子:1.849)

37.Meng Q, Zhang Y, Liu XQ. rare group I intron sequence element in a bacterial ribonucleotide reductase gene. J Bacteriol. 2007, 189, 5, 2150-2154(SCI,影响因子:4.149)

38.Meng Q, Wang Y, Liu XQ. An intron-encoded maturase splicing multiple introns including four group II introns in a cyanobacterial dnaN gene. J Biol Chem. 2005, 280, 42, 35085-8(SCI,影响因子:6.849)

39.Yang J, Meng Q, Liu XQ. Intein harbouring large tandem repeats in replicative DNA helicase of Tri chodesmium erythraeum. Mol Microbiol. 2004, 51, 4, 1185-1192(SCI,影响因子:4.819)

40.Liu XQ, Yang J, Meng Q. Four inteins and three group II introns encoded in a bacterial ribonucleotide reductase gene. J Biol Chem. 2003, 278, 47, 46826-31(SCI,影响因子:6.849)

41.Qu Lianghu, Meng Q, Zhou H, Chen YQ. Identification of 10 novel SnoRNA gene clusters from Arabidopsis thaliana. Nucleic Acids Res. 2001, 29, 7, 1623-30(SCI,影响因子:9.112)


教学课程

分子生物学、分子生物学技术(为硕士研究生开设);生物工程现状与未来(为全校本科学生开设);生物化学与分子生物学综合实验(为生物材料本科学生开设)。


国际交流与合作

1.2002年至2004年,加拿大Dalhousie大学医学院,博士后工作;

2.2006年12月至2007年2月,加拿大Dalhousie大学医学院,高级访问学者;

3.2011年11月至2012年3月,美国Weill Cornell Medical College,高级访问学者;

4.2012年7月至2012年8月,瑞典Karolinska Institutet,高级访问学者;

5.2013年7月至2013年8月,瑞典Karolinska Institutet、Sveriges lantbruksuniversitet, 高级访问学者;

6.2014年7月至2014年8月,瑞典Karolinska Institutet、Sveriges lantbruksuniversitet, 高级访问学者;

7.2015年8月至2015年9月,美国Weill Cornell Medical College,高级访问学者;

8.2018年7月15日至2018年8月15日,加拿大Dalhousie大学医学院,高级访问学者;

9.2019年1月15日至2019年1月30日,新加坡National University of Singapore,高级访问学者;

10.2019年7月12日至2019年8月12日,瑞典Karolinska Institutet,高级访问学者;  

11.2020年1月14日至2020年2月4日,德国University of Bayreuth,高级访问学者。 


联系电话:021-67792651            E-MAIL:mengqing@dhu.edu.cn